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Genome-Wide Association Mapping To Approach The Candidate Gene Having Potential Role In Dairy Bull Fertility

by Asif Nadeem | Prof.Dr.Masroor Elahi Babar | Dr.Atif Hanif | Prof.Dr.Muhammad Abdullah.

Material type: book Book; Format: print Publisher: 2011Dissertation note: Reproductive efficiency is a most important determinant of dairy profitability. Fertility in the herd is absolutely critical for both male and female animals. Fertility studies in dairy cattle were directed toward the female side and very little importance has been placed on the influence of the service bull. In this study association mapping was carried out Cor fertility trait in Holstein dairy cattle bulls using high-throughput and a high-density SNi> genotyping array. Single nucleotide polymorphisms (SNPs) were associated with dair, cattle bull fertility. Associated SNPs were queried in the bovine genome. Seven SNPs were found within the genes and fourteen were within 10 kh o! a gene. Seven gl'nes. namely LEPRELl, MOBKL3, CD247, LRRC8J\, LRFN5, IT] I [J and [·.NTP[) J were seleeted as candidate genes. Resequencing and fine mapping of selected candidate genes were performed and identified SNPs were associated with dairy cattle hull fertility. This is the first GWA study for dairy bull fertility using the Illumina Bovine S~ P50 Bcadchip containing 54001 S Ps powered by I1lumina lnfinium-Il assay. Availability: Items available for loan: UVAS Library [Call number: 1354,T] (1).

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Identification Of Molecular Markers In Bmp15 Gene Of Different Pakistan Sheep And Goat Breeds

by Ahmad Nawaz | Prof.Dr.Masroor Elahi Babar | Prof. Dr | Prof. Dr. Khalid Javed.

Material type: book Book; Format: print ; Nature of contents: biography Publisher: 2011Dissertation note: Genetics of prolificacy in sheep and goat emphasize the importance of main genes which have been made known to affect litter size and rate of ovulation through various mechanisms. Natural mutations in prolific sheep and goat breeds have shown that the transforming growth factor beta (TGF-?) super family ligands such as bone morphogenetic protein 15 is crucial for ovulation and as well as for increasing litter size. Keeping in view the importance of prolificacy in sheep and goat, a research project was planed to identify the polymorphism, its association with fecundity and uncovering the nucleotide picture of BMP15 fecundity gene in sheep and goat breeds of Pakistan. In the research finding, various polymorphism, insertion and deletion of nucleotides in goat and sheep breeds of Pakistan were identified and associated with fecundity and secondly, some novel polymorphism in Pakistani goat and sheep breeds were identified which are different from the goat and sheep breeds of the world. This is the first report of the whole nucleotide of BMP15 gene in the sheep. A lot of work has been reported on these genes but total nucleotide picture in the sheep is not reported. Sequences of Bmp15 gene from sheep and goat breeds of Pakistan has been submitted to the NCBI GenBank database libraries,USA under accession numbers JN655669, JN655670, JN655671 and JN655672. It will result in fast vertical expansion of small ruminants to increase the mutton production and uplift the socio economic condition of small ruminant's farmers in the country. Availability: Items available for loan: UVAS Library [Call number: 1421,T] (1).

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